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Anti-CACNG4 Antibody

CaVγ4, Voltage-dependent calcium channel gamma-4 subunit, TARP gamma-4, Transmembrane AMPAR regulatory protein gamma-4

Cat #: ACC-114
Alternative Name CaVγ4, Voltage-dependent calcium channel gamma-4 subunit, TARP gamma-4, Transmembrane AMPAR regulatory protein gamma-4
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)EKNKELRFKTKRE, corresponding to amino acid residues 208-220 of rat CACNG4 (Accession Q8VHW9). Intracellular, C-terminus.
Accession (Uniprot) Number Q8VHW9
Gene ID 140725
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human - identical.
RRID AB_2340908.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, wb
May also work in: ih*, ip*
Western blot
  • Western blot analysis of rat brain (lanes 1 and 5), mouse brain (lanes 2 and 6), human SH-SY5Y brain neuroblastoma (lanes 3 and 7) and  rat PC12 pheochromocytoma adrenal gland (lanes 4 and 8) lysates:
    Western blot analysis of rat brain (lanes 1 and 5), mouse brain (lanes 2 and 6), human SH-SY5Y brain neuroblastoma (lanes 3 and 7) and  rat PC12 pheochromocytoma adrenal gland (lanes 4 and 8) lysates:
    1-4. Anti-CACNG4 Antibody (#ACC-114), (1:800).
    5-8. Anti-CACNG4 Antibody, preincubated with CACNG4 Blocking Peptide (#BLP-CC114).
Immunocytochemistry
  • Human islet beta cells (see Luan, C. et al. (2019) Commun. Biol. 2, 106.).

References
  1. Tsunemi, T. et al. (2002) J. Biol. Chem. 277, 7214.
  2. Scheuber, A. et al. (2004) J. Neurosci. 24, 10402.
  3. Burgess, D.L. et al. (1999) Genome Res. 9, 1204.
  4. Korber, C. et al. (2007) J. Neurosci. 27, 8442.
Scientific background

Voltage dependent Ca2+ channels (CaV channels) are pivotal players in many physiological roles such as secretion, contraction migration and excitation1.

They are composed of several subunits; α1, β, α2δ and γ. CaV channels were originally divided into six physiological types: L, N, P, Q, R, and T type2.

L-type calcium channels are composed of five subunits. CaVγ4, represents one of these subunits, and is one of several γ subunit proteins. It is an integral membrane protein that is thought to stabilize the Ca2+ channel in an inactive (closed) state. The encoding gene is a member of the neuronal Ca2+ channel γ subunit gene subfamily of the PMP-22/EMP/MP20 family and is located in a cluster with two similar gamma subunit-encoding genes3.

CaVγ4, along with CaVγ2, CaVγ3 and CaVγ8 have been associated with AMPA receptors and have been shown to modulate the trafficking of receptors to the plasma membrane as well as electrophysiological key properties4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 08/06/2021

Anti-CACNG4 Antibody (#ACC-114) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunocytochemistry applications. It has been designed to recognize CaVγ4 from rat, mouse, and human samples.

For research purposes only, not for human use
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