Overview
Type: Synthetic peptide
Form: Lyophilized powder
Cav3.2/CACNA1H Blocking Peptide (#BLP-CC025) is the original antigen used for immunization during Anti-CaV3.2 (CACNA1H) Antibody (#ACC-025) generation. The blocking peptide binds and ‘blocks’ Anti-Cav3.2/CACNA1H primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat DRG lysates:1. Anti-CaV3.2 (CACNA1H) Antibody (#ACC-025), (1:200).
2. Anti-CaV3.2 (CACNA1H) Antibody, preincubated with Cav3.2/CACNA1H Blocking Peptide (#BLP-CC025).
Expression of CaV3.2 in mouse cerebellumImmunohistochemical staining of mouse cerebellum frozen sections with Anti-CaV3.2 (CACNA1H) Antibody (#ACC-025), (1:100). A. CaV3.2 appears adjacent to Purkinje cells and in fibers in the molecular layer (red). B. Staining of Purkinje cells with mouse anti-parvalbumin (PV, green). C. Merged image of panels A and B demonstrates presence of CaV3.2 adjacent to Purkinje cells.
Expression of CaV3.2 in rat DRGImmunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections with Anti-CaV3.2 (CACNA1H) Antibody (#ACC-025), (1:50). Staining is specific for DRG. Note that neither glial cells nor axonal fibers are stained. Hoechst 33342 is used as the counterstain.
Properties
Sequence
- CHVEGPQERARVAHS, corresponding to amino acid residues 581-595 of rat CaV3.2 (Accession Q9EQ60).
Accession (Uniprot) Number Q9EQ60
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
