Overview
Cat #: BLP-CC025
Type: Synthetic peptide
Form: Lyophilized powder
Cav3.2/CACNA1H Blocking Peptide (#BLP-CC025) is the original antigen used for immunization during Anti-CaV3.2 (CACNA1H) Antibody (#ACC-025) generation. The blocking peptide binds and ‘blocks’ Anti-Cav3.2/CACNA1H primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
Application key:
WB-western blot, IHC-Immunohistochemistry
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of rat DRG lysates:1. Anti-CaV3.2 (CACNA1H) Antibody (#ACC-025), (1:200).
2. Anti-CaV3.2 (CACNA1H) Antibody, preincubated with Cav3.2/CACNA1H Blocking Peptide (#BLP-CC025). - Expression of CaV3.2 in mouse cerebellumImmunohistochemical staining of mouse cerebellum frozen sections with Anti-CaV3.2 (CACNA1H) Antibody (#ACC-025), (1:100). A. CaV3.2 appears adjacent to Purkinje cells and in fibers in the molecular layer (red). B. Staining of Purkinje cells with mouse anti-parvalbumin (PV, green). C. Merged image of panels A and B demonstrates presence of CaV3.2 adjacent to Purkinje cells.
- Expression of CaV3.2 in rat DRGImmunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections with Anti-CaV3.2 (CACNA1H) Antibody (#ACC-025), (1:50). Staining is specific for DRG. Note that neither glial cells nor axonal fibers are stained. Hoechst 33342 is used as the counterstain.
Properties
Sequence
Accession (Uniprot) Number Q9EQ60
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
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