CCR7 (extracellular) Blocking Peptide (#BLP-CR027) is the original antigen used for immunization during Anti-CCR7 (extracellular) Antibody (#ACR-027) generation. The blocking peptide binds and ‘blocks’ Anti-CCR7 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of human T-cell leukemia Jurkat cell line (lanes 1 and 4), human acute lymphoblastic leukemia MOLT-4 (lanes 2 and 5) and mouse macrophage J774.1 (lanes 3 and 6) cell lysates:
- Expression of CCR7 in mouse deep cerebellar nuclei.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-CCR7 (extracellular) Antibody (#ACR-027), (1:400), followed by goat anti-rabbit-AlexaFluor-488. A. CCR7 immunoreactivity (green) appears in blood vessels (arrows). B. Pre-incubation of the antibody with CCR7 (extracellular) Blocking Peptide (#BLP-CR027), suppressed staining. Cell nuclei are stained with DAPI (blue).
- (C)DEVTDDYIGDNTTVD, corresponding to amino acid residues 26-40 of human CCR7 (Accession P32248).