CFTR Blocking Peptide (#BLP-CL006) is the original antigen used for immunization during Anti-CFTR Antibody (#ACL-006) generation. The blocking peptide binds and ‘blocks’ Anti-CFTR primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat lung membranes:
- Expression of CFTR in rat lungsImmunohistochemical staining of rat lungs sections using Anti-CFTR Antibody (#ACL-006) (left panel). Strong staining of bronchial epithelial cells (red) and lighter staining of alveolar cells (red-brown) is apparent. There is also positive staining of macrophages while smooth muscle and endothelium are negative. Counterstain of cell nuclei appears blue. A negative control is shown in the right panel.
- (C)KEETEEEVQDTRL, corresponding to amino acid residues 1468-1480 of human CFTR (Accession P13569).