Overview
Type: Synthetic peptide
Form: Lyophilized powder
CFTR Blocking Peptide (#BLP-CL006) is the original antigen used for immunization during Anti-CFTR Antibody (#ACL-006) generation. The blocking peptide binds and ‘blocks’ Anti-CFTR primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat lung membranes:1. Anti-CFTR Antibody (#ACL-006), (1:200).
2. Anti-CFTR Antibody, preincubated with CFTR Blocking Peptide (#BLP-CL006).
Expression of CFTR in rat lungsImmunohistochemical staining of rat lungs sections using Anti-CFTR Antibody (#ACL-006) (left panel). Strong staining of bronchial epithelial cells (red) and lighter staining of alveolar cells (red-brown) is apparent. There is also positive staining of macrophages while smooth muscle and endothelium are negative. Counterstain of cell nuclei appears blue. A negative control is shown in the right panel.
Properties
Sequence
- (C)KEETEEEVQDTRL, corresponding to amino acid residues 1468-1480 of human CFTR (Accession P13569).
Accession (Uniprot) Number P13569
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
