CHERP Blocking Peptide (#BLP-CC330) is the original antigen used for immunization during Anti-CHERP Antibody (#ACC-330) generation. The blocking peptide binds and ‘blocks’ Anti-CHERP primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and mouse C2C12 muscle myoblast cell line (lanes 3 and 6) lysates:
- Expression of CHERP in mouse brainImmunohistochemical staining of mouse brain sections using Anti-CHERP Antibody (#ACC-330). A. In mouse hippocampal CA3 region, CHERP immunoreactivity (green) appears in neurons (arrows) of CA3 pyramidal layer (P). B. In parietal cortex, CHERP staining (green) is detected in neurons (arrows). Nuclei are demonstrated using DAPI as the counterstain (blue).
- (C)KLEDHEYKPLDPKDIR, corresponding to amino acid residues 669-684 of mouse CHERP (Accession Q8CGZ0).