Overview
Type: Synthetic peptide
Form: Lyophilized powder
CHERP Blocking Peptide (#BLP-CC330) is the original antigen used for immunization during Anti-CHERP Antibody (#ACC-330) generation. The blocking peptide binds and ‘blocks’ Anti-CHERP primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and mouse C2C12 muscle myoblast cell line (lanes 3 and 6) lysates:1-3. Anti-CHERP Antibody (#ACC-330), (1:1500).
4-6. Anti-CHERP Antibody, preincubated with CHERP Blocking Peptide (#BLP-CC330).
Expression of CHERP in mouse brainImmunohistochemical staining of mouse brain sections using Anti-CHERP Antibody (#ACC-330). A. In mouse hippocampal CA3 region, CHERP immunoreactivity (green) appears in neurons (arrows) of CA3 pyramidal layer (P). B. In parietal cortex, CHERP staining (green) is detected in neurons (arrows). Nuclei are demonstrated using DAPI as the counterstain (blue).
Properties
Sequence
- (C)KLEDHEYKPLDPKDIR, corresponding to amino acid residues 669-684 of mouse CHERP (Accession Q8CGZ0).
Accession (Uniprot) Number Q8CGZ0
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
