CHRM1 (443-458) Blocking Peptide (#BLP-MR010) is the original antigen used for immunization during Anti-CHRM1 (443-458) Antibody (#AMR-010) generation. The blocking peptide binds and ‘blocks’ Anti-CHRM1 (443-458) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain lysate:
- Expression of Muscarinic acetylcholine receptor M1 in mouse striatumImmunohistochemical staining of mouse striatum using Anti-CHRM1 (443-458) Antibody (#AMR-010), (1:100). M1 mAChR staining (red) appears in the matrix (M), not in the patches (P). Blood vessels in the matrix (arrow) are also stained. DAPI is used as the counterstain (blue).
- (C)RKIPKRPGSVHRTPSR, corresponding to amino acid residues 443-458 of human M1 muscarinic receptor (Accession P11229).