Overview
Type: GST fusion protein
Form: Lyophilized powder
CHRM1 Blocking Peptide (#BLP-MR001) is the original antigen used for immunization during Anti-CHRM1 Antibody (#AMR-001) generation. The blocking peptide binds and ‘blocks’ Anti-CHRM1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-CHRM1 Antibody (#AMR-001), (1:200).
2. Anti-CHRM1 Antibody, preincubated with CHRM1 Blocking Peptide (#BLP-MR001).
Expression of Muscarinic acetylcholine receptor M1 in rat striatumImmunohistochemical staining of rat striatum (ST) using Anti-CHRM1 Antibody (#AMR-001). A. M1 mAChR appears in the striatum (green). B. Staining of interneurons with mouse anti-parvalbumin (PV, red). C. Confocal merge of M1 mAChR and PV demonstrates localization of PV expressing neurons in the striatal matrix; not in striatal patches (P).
Expression of Muscarinic acetylcholine receptor M1 in mouse striatumImmunohistochemical staining of mouse striatum (ST) using Anti-CHRM1 Antibody (#AMR-001). A. M1 mAChR appears in the striatum (green). B. Staining of interneurons with mouse anti-parvalbumin (PV, red). C. Confocal merge of M1 mAChR and PV demonstrates localization of PV expressing neurons in the striatal matrix; not in striatal patches (P).- Human colon (1:50) (Harrington, A.M. et al. (2010) Neurogastroenterol. Motil. 22, 999.).
Properties
Sequence
- GST fusion protein with the sequence GSETPGKGGGSSSSSERSQPGAEGSPETPPGRCCRCCRAPRLLQAYSWKEEEEEDEGSMESLTSSEGEEPGSEVVIKMPMVDPEAQAPTKQPPRSSPNTVKRPTKKGRDRAGKGQKPRGKEQLAKRK, corresponding to amino acid residues 227-353 of human M1 (Accession P11229).
Accession (Uniprot) Number P11229
Peptide Confirmation Confirmed by DNA sequence and SDSPAGE.
Purity >95% (SDS-PAGE)
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 μl PBS.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 3 μg fusion protein per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
