Overview
Type: GST fusion protein
Form: Lyophilized powder
CHRM2 Blocking Peptide (#BLP-MR002) is the original antigen used for immunization during Anti-CHRM2 Antibody (#AMR-002) generation. The blocking peptide binds and ‘blocks’ Anti-CHRM2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-CHRM2 Antibody (#AMR-002), (1:200).
2. Anti-CHRM2 Antibody, preincubated with CHRM2 Blocking Peptide (#BLP-MR002).
Expression of Muscarinic acetylcholine receptor M2 in mouse parieto-temporal cortex sectionsImmunohistochemical staining of mouse parieto-temporal cortex frozen sections (non-consecutive) using Anti-GIRK2 (Kir3.2) Antibody (#APC-006), (1:100) and Anti-CHRM2 Antibody (#AMR-002), (1:100). mAChR M2 staining (red) was dense in layer IV, with fibers climbing to layers II-III. Kir3.2 K+ channel staining (green) was dense in layers IV and I. Overlapping expression of Kir3.2 channel and mAChR M2 is seen in cortical layers.- Human colon (1:50) (Harrington, A.M. et al. (2010) Neurogastroenterol. Motil. 22, 999.).
Properties
Sequence
- GST fusion protein with the sequence VANQDPVSPSLVQGRIVKPN NNNMPSSDDGLEHNKIQNGKAPRDPVTENCVQGEEKESSNDSTSV SAVASNMRDDEITQDENTVSTSLGHSKDENSKQTCIRIGTKTPKS DSCTPTNTTVEVVGSSGQNGDE, corresponding to amino acid residues 225-356 of human M2 (Accession P08172).
Accession (Uniprot) Number P08172
Peptide Confirmation Confirmed by DNA sequence and SDSPAGE.
Purity >95% (SDS-PAGE)
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 μl PBS.
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 3 µg fusion protein per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
