CHRM3 Blocking Peptide (#BLP-MR006) is the original antigen used for immunization during Anti-CHRM3 Antibody (#AMR-006) generation. The blocking peptide binds and ‘blocks’ Anti-CHRM3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain lysate:
- Human colon (1:50) (Harrington, A.M. et al. (2010) Neurogastroenterol. Motil. 22, 999.).
- Expression of CHRM3 in mouse cortex.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-CHRM3 Antibody (#AMR-006), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. CHRM3 immunoreactivity (green) appears in neurons (arrows). B. Pre-incubation of the antibody with CHRM3 Blocking Peptide (#BLP-MR006), suppressed staining. Cell nuclei are stained with DAPI (blue).
- (C)TLAKRFALKTRSQITKRKR, corresponding to amino residues 461-479 of rat M3 (Accession P08483).