CLIC1 Blocking Peptide (#BLP-CL021) is the original antigen used for immunization during Anti-CLIC1 Antibody (#ACL-021) generation. The blocking peptide binds and ‘blocks’ Anti-CLIC1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of human colorectal adenocarcinoma HT-29 (lanes 1 and 5), transplantable human carcinoma cell line T-84 (lanes 2 and 6), human pancreatic carcinoma PANC-1 (lanes 3 and 7) and rat kidney (lanes 4 and 8) lysates:
- (C)RGFTIPEAFRGVHR, corresponding to amino acid residues 195-208 of human CLIC1 (Accession O00299).