Connexin-36 Blocking Peptide (#BLP-CC209) is the original antigen used for immunization during Anti-Connexin-36 Antibody (#ACC-209) generation. The blocking peptide binds and ‘blocks’ Anti-Connexin-36 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:
- Expression of Connexin-36 in rat spinal cordImmunohistochemical staining of perfusion-fixed frozen rat spinal cord sections using Anti-Connexin-36 Antibody (#ACC-209), (1:300). Cx36 staining (in red) appears in processes (arrows) along the first layer of the dorsal horn of the rat spinal cord. Cell nuclei are stained with DAPI (blue).
- (C)EVKELAPHPSGLR, corresponding to amino acid residues 170-182 of rat Connexin-36 (Accession O70610).