Contactin-1 (extracellular) Blocking Peptide (#BLP-NR171) is the original antigen used for immunization during Anti-Contactin-1/CNTN1 (extracellular) Antibody (#ANR-171) generation. The blocking peptide binds and ‘blocks’ Anti-Contactin-1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
- Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:
- Expression of Contactin-1 in rat cerebral ventricle:Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Contactin-1/CNTN1 (extracellular) Antibody (#ANR-171), (1:200), followed by goat anti-rabbit-AlexaFluor-488. Contactin-1 immunoreactivity (green) appears in ependymal cells (arrows) lining the lateral cerebral ventricle (LV). Cell nuclei are stained with DAPI (blue).
- (C)RPDGQHDGKLFSTHK, corresponding to amino acid residues 947 - 961 of rat Contactin-1 (Accession Q63198).