CPLX1/2 Blocking Peptide (#BLP-NR011) is the original antigen used for immunization during Anti-CPLX1/2 Antibody (#ANR-011) generation. The blocking peptide binds and ‘blocks’ Anti-CPLX1/2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
- Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:
- (C)KDMGKMLGGDEEKD, corresponding to amino acid residues 14-27 of rat Complexin-1 (Accession P63041, P84087).