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Anti-CPLX1/2 Antibody

Complexin 1, Complexin I, Synaphin-2, Complexin 2, Complexin II, Synaphin-1

Cat #: ANR-011
Alternative Name Complexin 1, Complexin I, Synaphin-2, Complexin 2, Complexin II, Synaphin-1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)KDMGKMLGGDEEKD, corresponding to amino acid residues 14-27 of rat Complexin-1 (Accession P63041) and rat Complexin-2 (Accession P84087). Intracellular, N-terminus.
Accession (Uniprot) Number P63041, P84087
Gene ID 64832, 116657
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Complexin 1: Mouse, human – identical.
Complexin 2: Rat, mouse, human – 13/14 amino acid residues identical.
RRID AB_2756683.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity The antibody will recognize both Complexin-1 and Complexin-2.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ih*, ip*
Western blot
  • Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
    Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
    1,2. Anti-CPLX1/2 Antibody (#ANR-011), (1:200).
    3,4. Anti-CPLX1/2 Antibody, preincubated with CPLX1/2 Blocking Peptide (#BLP-NR011).
  • Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:
    Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:
    1. Anti-CPLX1/2 Antibody (#ANR-011), (1:200).
    2. Anti-CPLX1/2 Antibody, preincubated with CPLX1/2 Blocking Peptide (#BLP-NR011).
References
  1. Gong, J. et al. (2016) Proc. Natl. Acad. Sci. U.S.A. 113, 47.
  2. Choi, U.B. et al. (2016) Elife 5, e16886.
  3. Mohrmann, R. et al. (2015) Cell. Mol. Life. Sci. 72, 4221.
Scientific background

Complexins (1 through 4) are small cytosolic proteins that bind tightly to a complex of SNARE proteins to regulate synaptic vesicle membrane fusion via its interaction with synaptotagmin. Complexins activate the quick release of neurotransmitters, triggered by calcium release. They also regulate the “spontaneous release” of neurotransmitters1-3.

Complexins are evolutionarily conserved in mammals, Drosophila, and Caenorhabditis elegans1,2. They are divided into four structural domains: flexible N- and C-terminal domains, an accessory domain, and a central α-helical domain. The N-terminal domain plays a role in activation of fast synchronous release in murine neurons and in isolated chromaffin cells. The C-terminal domain binds to phospholipids and is important for regulating spontaneous release in neuronal cultures and suppressing Ca2+-independent fusion in vitro. The accessory domain is necessary for regulating spontaneous release and the central domain is required for all functions1,2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 02/08/2022

Anti-CPLX1/2 Antibody (#ANR-011) is an antibody directed against epitopes of rat Complexin 1 and Complexin 2. The antibody can be used in western blot analysis. It has been designed to recognize both Complexin 1 and Complexin 2 from human, rat, and mouse samples.

For research purposes only, not for human use
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