Cat #: BLP-PR062
Type: Synthetic peptide
Form: Lyophilized powder
A Blocking peptide for Anti-CRTH2/GPR44 (extracellular) Antibody (#APR-062). Intended to be used as a negative control.
For research purposes only, not for human use
- Western blot analysis of rat brain lysate:
- Western blot analysis of mouse brain lysate:
- Expression of DP2 receptor (CRTH2/GPR44) in rat medial septum and mouse cerebellumImmunohistochemical staining of perfusion-fixed frozen rat and mouse brain sections with Anti-CRTH2/GPR44 (extracellular) Antibody (#APR-062), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. A. DP2 receptor staining (green) in rat brain sections is detected in neuron-like profiles (arrows) in the medial septum/diagonal band. B. DP2 receptor staining (green) in mouse brain is observed in Purkinje cells (horizontal arrow) and in a subset of neurons in the granule layer (GL, vertical arrow). Cell nuclei are stained with DAPI (blue).
- (C)RAHSVTTLRQLASR, corresponding to amino acid residues 270-283 of rat PTGDR2 (Accession Q6XKD3).
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
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