CRTH2/GPR44 (extracellular) Blocking Peptide (#BLP-PR062) is the original antigen used for immunization during Anti-CRTH2/GPR44 (extracellular) Antibody (#APR-062) generation. The blocking peptide binds and ‘blocks’ Anti-CRTH2/GPR44 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain lysate:
- Western blot analysis of mouse brain lysate:
- Expression of DP2 receptor (CRTH2/GPR44) in rat medial septum and mouse cerebellumImmunohistochemical staining of perfusion-fixed frozen rat and mouse brain sections with Anti-CRTH2/GPR44 (extracellular) Antibody (#APR-062), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. A. DP2 receptor staining (green) in rat brain sections is detected in neuron-like profiles (arrows) in the medial septum/diagonal band. B. DP2 receptor staining (green) in mouse brain is observed in Purkinje cells (horizontal arrow) and in a subset of neurons in the granule layer (GL, vertical arrow). Cell nuclei are stained with DAPI (blue).
- (C)RAHSVTTLRQLASR, corresponding to amino acid residues 270-283 of rat PTGDR2 (Accession Q6XKD3).