Anti-Prostanoid DP2 Receptor (extracellular)

Prostaglandin D2 Receptor 2, CRTH2, GPR44, CD294, PTGDR2
    Cat #: APR-062
    Sizes: 25 µl, 50 µl, 0.2 ml
  • Lyophilized Powder
  • Antigen Incl.
  • QC Tested
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: m, r
    Immunogen
    Peptide (C)RAHSVTTLRQLASR, corresponding to amino acid residues 270-283 of rat Prostanoid DP2 receptor(Accession Q6XKD3). 3rd extracellular loop.
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse – identical. Will not recognize DP2 receptor from human samples.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Specificity Will not recognize DP2 receptor from human samples.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ifc, ih, wb
    May also work in: ic*, ip*, lci*
    Western blot
    Western blot analysis of rat brain lysate:
    1. Anti-Prostanoid DP2 Receptor (extracellular) antibody (#APR-062), (1:200).
    2. Anti-Prostanoid DP2 Receptor (extracellular) antibody, preincubated with the control peptide antigen.
    Western blot analysis of mouse brain lysate:
    1. Anti-Prostanoid DP2 Receptor (extracellular) antibody (#APR-062), (1:400).
    2. Anti-Prostanoid DP2 Receptor (extracellular) antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Expression of DP2 Receptor in rat medial septum and mouse cerebellum
    Immunohistochemical staining of perfusion-fixed frozen rat and mouse brain sections with Anti-Prostanoid DP2 Receptor (extracellular) antibody (#APR-062), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. A. DP2 Receptor staining (green) in rat brain sections is detected neuron-like profiles (arrows) in the medial septum/diagonal band. B. DP2 Receptor staining (green) in mouse brain is observed in purkinje cells (horizontal arrow) and of a subset of neurons in the granule layer (GL, vertical arrow). Cell nuclei are stained with DAPI (blue).
    Indirect flow cytometry
    Indirect flow cytometry of live intact mouse P815 mast cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-Prostanoid DP2 Receptor (extracellular) antibody (#APR-062), (5 µg) + goat-anti-rabbit-FITC.
    References
    1. Hirata, T. and Narumiya, S. (2011) Chem. Rev. 111, 6209.
    Scientific background

    Prostanoids are potent oxygenated lipid molecules that contribute to a wide variety of physiological responses and pathological processes. There are currently five primary bioactive types of prostanoids: PGD2, PGE2, PGF2R, PGI2, and thromboxane A2 (TXA2).

    All prostanoid receptors belong to the G-protein coupled receptor superfamily. Unlike other receptors of this family the Prostaglandin D2 Receptor (also known as CRTH2, GPR44) is structurally distinct and belongs to the family of leukocyte chemoattractant receptors.

    The DP2 receptor is expressed in Th2 cells, basophils, and eosinophils, and it mediates the chemotaxis of these cells in response to PGD2. The ability of PGD2 to promote eosinophil accumulation in the airways is mimicked by selective DP2 agonists but not DP1 agonists.

    Much effort has been directed toward developing DP2 agonists and antagonists. Indomethacin and ramatroban, which act, respectively, as an agonist and an antagonist, were the first nonprostanoid compounds to be identified as DP2 ligands. These two compounds have frequently used as leads for identifying new selective compounds1.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 25/12/2017

    Anti-Prostanoid DP2 Receptor (extracellular) (#APR-062) is a highly selective antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope, and could potentially be used for detecting the protein in living cells. It has been designed to recognize DP2 Receptor from mouse and rat samples. It is unlikely to recognize the receptor from human samples.

    For research purposes only, not for human use