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Anti-CRTH2/GPR44 (extracellular) Antibody

Prostanoid DP2 receptor, Prostaglandin D2 receptor 2, PTGDR2, CD294

Cat #: APR-062
Alternative Name Prostanoid DP2 receptor, Prostaglandin D2 receptor 2, PTGDR2, CD294
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
Immunogen
  • Peptide (C)RAHSVTTLRQLASR, corresponding to amino acid residues 270-283 of rat PTGDR2 (Accession Q6XKD3). 3rd extracellular loop.
Accession (Uniprot) Number Q6XKD3
Gene ID 309212
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – identical. Will not recognize DP2 receptor from human samples.
RRID AB_2756760.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity Will not recognize DP2 receptor from human samples.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ifc, ih, lci, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of rat brain lysate:
    Western blot analysis of rat brain lysate:
    1. Anti-CRTH2/GPR44 (extracellular) Antibody (#APR-062), (1:200).
    2. Anti-CRTH2/GPR44 (extracellular) Antibody, preincubated with CRTH2/GPR44 (extracellular) Blocking Peptide (#BLP-PR062).
  • Western blot analysis of mouse brain lysate:
    Western blot analysis of mouse brain lysate:
    1. Anti-CRTH2/GPR44 (extracellular) Antibody (#APR-062), (1:400).
    2. Anti-CRTH2/GPR44 (extracellular) Antibody, preincubated with CRTH2/GPR44 (extracellular) Blocking Peptide (#BLP-PR062).
Immunohistochemistry
  • Expression of DP2 receptor (CRTH2/GPR44) in rat medial septum and mouse cerebellum
    Expression of DP2 receptor (CRTH2/GPR44) in rat medial septum and mouse cerebellum
    Immunohistochemical staining of perfusion-fixed frozen rat and mouse brain sections with Anti-CRTH2/GPR44 (extracellular) Antibody (#APR-062), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. A. DP2 receptor staining (green) in rat brain sections is detected in neuron-like profiles (arrows) in the medial septum/diagonal band. B. DP2 receptor staining (green) in mouse brain is observed in Purkinje cells (horizontal arrow) and in a subset of neurons in the granule layer (GL, vertical arrow). Cell nuclei are stained with DAPI (blue).
Indirect flow cytometry
  • Cell surface detection of CRTH2 in live intact mouse P815 mast cells:
    Cell surface detection of CRTH2 in live intact mouse P815 mast cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-CRTH2/GPR44 (extracellular) Antibody (#APR-062), (5 µg) + goat-anti-rabbit-FITC.
References
  1. Hirata, T. and Narumiya, S. (2011) Chem. Rev. 111, 6209.
Scientific background

Prostanoids are potent oxygenated lipid molecules that contribute to a wide variety of physiological responses and pathological processes. There are currently five primary bioactive types of prostanoids: PGD2, PGE2, PGF2R, PGI2, and thromboxane A2 (TXA2).

All prostanoid receptors belong to the G-protein coupled receptor superfamily. Unlike other receptors of this family the Prostaglandin D2 receptor (also known as CRTH2, GPR44) is structurally distinct and belongs to the family of leukocyte chemoattractant receptors.

The DP2 receptor is expressed in Th2 cells, basophils, and eosinophils, and it mediates the chemotaxis of these cells in response to PGD2. The ability of PGD2 to promote eosinophil accumulation in the airways is mimicked by selective DP2 agonists but not DP1 agonists.

Much effort has been directed toward developing DP2 agonists and antagonists. Indomethacin and ramatroban, which act, respectively, as an agonist and an antagonist, were the first nonprostanoid compounds to be identified as DP2 ligands. These two compounds have frequently used as leads for identifying new selective compounds1.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 12/08/2021

Anti-CRTH2/GPR44 (extracellular) Antibody (#APR-062) is a highly selective antibody directed against an epitope of the rat Prostanoid DP2 receptor. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. The antibody recognizes an extracellular epitope and can potentially be used for detecting the protein in living cells. It has been designed to recognize DP2 receptor from mouse and rat samples. It is unlikely to recognize the receptor from human samples.

For research purposes only, not for human use
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