DLGAP1/GKAP Blocking Peptide (#BLP-PZ041) is the original antigen used for immunization during Anti-DLGAP1/GKAP Antibody (#APZ-041) generation. The blocking peptide binds and ‘blocks’ Anti-DLGAP1/GKAP primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
- Expression of GKAP in rat hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-DLGAP1/GKAP Antibody (#APZ-041), (1:200), followed by goat anti-rabbit-AlexaFluor-488. GKAP staining (green) in hippocampal CA3 region is detected in neurons (arrows) of CA3 pyramidal layer (P). Cell nuclei are stained with DAPI (blue).
- (C)KISNEHSPKLQIRSH, corresponding to amino acid residues 397-411 of rat DLGAP1 (Accession P97836).