Overview
Type: Synthetic peptide
Form: Lyophilized powder
DPP10 (extracellular) Blocking Peptide (#BLP-PC147) is the original antigen used for immunization during Anti-DPP10 (extracellular) Antibody (#APC-147) generation. The blocking peptide binds and ‘blocks’ Anti-DPP10 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysate:1,2. Anti-DPP10 (extracellular) Antibody (#APC-147), (1:400).
3,4. Anti-DPP10 (extracellular) Antibody, preincubated with DPP10 (extracellular) Blocking Peptide (#BLP-PC147).
Western blot analysis of human SH-SY5Y neuroblastoma (lane 1 and 3) and human HT-29 colon adenocarcinoma (lanes 2 and 4) cell line lysate:1,2. Anti-DPP10 (extracellular) Antibody (#APC-147), (1:200).
3,4. Anti-DPP10 (extracellular) Antibody, preincubated with DPP10 (extracellular) Blocking Peptide (#BLP-PC147).
Expression of DPP10 in mouse cerebellum and hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-DPP10 (extracellular) Antibody (#APC-147), (1:1000), followed by goat anti-rabbit-AlexaFluor-488. A. DPP10 immunoreactivity (green) in mouse cerebellum is detected in the Purkinje cell layer (P) in Purkinje cells (arrows) and diffusely in the molecular layer (MOL). B. DPP10 staining (green) in mouse hippocampal CA1 region is observed in the pyramidal layer (P, vertical arrow) and interneurons in the stratum oriens (SO, horizontal arrows). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- CSRKYEMTSDTWISK, corresponding to amino acid residues 356-370 of rat DPP10 (Accession Q6Q629).
Accession (Uniprot) Number Q6Q629
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
