Anti-DPP10 (extracellular) Antibody

Inactive dipeptidyl peptidase 10, Dipeptidyl peptidase X, DPP X, DPPY, DPL2, DPRP3
    Cat #: APC-147
    Alternative Name Inactive dipeptidyl peptidase 10, Dipeptidyl peptidase X, DPP X, DPPY, DPL2, DPRP3
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide CSRKYEMTSDTWISK, corresponding to amino acid residues 356-370 of rat DPP10 (Accession Q6Q629). Extracellular, C-terminus.
    • Anti-DPP10 (extracellular) Antibody
    Accession (Uniprot) Number Q6Q629
    Gene ID 363972
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse – 14/15 amino acid residues identical; human – 12/15 amino acid residues identical.
    RRID AB_2756746.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ih, wb
    May also work in: ic*, ifc*, ip*, lci*
    Western blot
    • Anti-DPP10 (extracellular) Antibody
      Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysate:
      1,2. Anti-DPP10 (extracellular) Antibody (#APC-147), (1:400).
      3,4. Anti-DPP10 (extracellular) Antibody, preincubated with the negative control antigen.
    • Anti-DPP10 (extracellular) Antibody
      Western blot analysis of human SH-SY5Y neuroblastoma (lane 1 and 3) and human HT-29 colon adenocarcinoma (lanes 2 and 4) cell line lysate:
      1,2. Anti-DPP10 (extracellular) Antibody (#APC-147), (1:200).
      3,4. Anti-DPP10 (extracellular) Antibody, preincubated with the negative control antigen.
    Immunohistochemistry
    • Anti-DPP10 (extracellular) Antibody
      Expression of DPP10 in mouse cerebellum and hippocampus
      Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-DPP10 (extracellular) Antibody (#APC-147), (1:1000), followed by goat anti-rabbit-AlexaFluor-488. A. DPP10 immunoreactivity (green) in mouse cerebellum is detected in the Purkinje cell layer (P) in Purkinje cells (arrows) and diffusely in the molecular layer (MOL). B. DPP10 staining (gren) in mouse hippocampal CA1 region is observed in the pyramidal layer (P, vertical arrow) and interneurons in the stratum oriens (SO, horizontal arrows). Cell nuclei are stained with DAPI (blue).
    References
    1. Bezerra, G.A. et al. (2015) Sci. Rep. 5, 8769.
    2. Cheng, C. et al. (2015) J. Comp. Neurol. 524, 846.
    3. McNicholas, K. et al. (2008) Clin. Chem. Lab. Med. 46, 262.
    Scientific background

    Dipeptidyl peptidase 10 (DPP10, DPPY) is a member of the prolyl oligopeptidase family (shares a 30% sequence identity with DPP4) and belongs to the S9B serine protease subfamily. DPP10 is a homologous glycosylated, single-pass type II transmembrane protein that lacks the crucial serine residue of the catalytic triad. Unlike other KV4 subunits such as DPP6, DPP10 has a restricted expression pattern including the brain, adrenal gland and pancreas.  The presence of DPP10 in endocrine cells suggests that the protein may also have an additional function related to the regulation of hormone secretion. DPP10 accelerates the activation and inactivation of KV4.3 channel gating with distinct biophysical properties.

    DPP10 associates with the α-subunits of the KV4 subfamily to form a ternary complex of approximately 750 kDa1. In addition, DPP10 forms complexes with other auxiliary subunits of the KV4 channels. In dorsal root ganglion DPP10 is co-localized with K+ channel-interacting proteins 1 and 2 (KChIPs) in the somatic surface and cytoplasm of KV4.3 nociceptors. Recently it has been hypothesized that KV4/KChIP/DPPL ternary complexes exist in subthreshold A-type K+ currents (Isas) expressing nociceptors and pain-modulating spinal interneurons2.

    DPP10 has been implicated in various pathologies. Aggregation of the protein is associated with neurodegenerative conditions such as Alzheimer’s, diffuse Lewy body disease and fronto-temporal dementia3.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-DPP10 (extracellular) Antibody (#APC-147) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the protein in living cells. It has been designed to recognize DPP10 from rat, mouse, and human samples.

    For research purposes only, not for human use
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