Overview
Type: Synthetic peptide
Form: Lyophilized powder
ENT1/SLC29A1 Blocking Peptide (#BLP-NT051) is the original antigen used for immunization during Anti-ENT1 (SLC29A1) Antibody (#ANT-051) generation. The blocking peptide binds and ‘blocks’ Anti-ENT1/SLC29A1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat kidney lysates (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):1,2. Anti-ENT1 (SLC29A1) Antibody (#ANT-051), (1:200).
3,4. Anti-ENT1 (SLC29A1) Antibody, preincubated with ENT1/SLC29A1 Blocking Peptide (#BLP-NT051).
Expression of ENT1 in rat DRGImmunohistochemical staining of rat frozen dorsal root ganglion (DRG) using Anti-ENT1 (SLC29A1) Antibody (#ANT-051), (1:100), followed by goat anti-rabbit-AlexaFluor- 555 secondary antibody. A. ENT1 labeling (red) appears in the cell bodies of the DRG. Note that the nerve fibers are not stained. B. Nuclear staining using DAPI as counterstain (blue). C. Merged images of A+B.
Properties
Sequence
- (C)EQETKLDLISKGEEPR, corresponding to amino acid residues 245-260 of human ENT1 (Accession Q99808).
Accession (Uniprot) Number Q99808
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
