ENT1/SLC29A1 Blocking Peptide (#BLP-NT051) is the original antigen used for immunization during Anti-ENT1 (SLC29A1) Antibody (#ANT-051) generation. The blocking peptide binds and ‘blocks’ Anti-ENT1/SLC29A1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat kidney lysates (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
- Expression of ENT1 in rat DRGImmunohistochemical staining of rat frozen dorsal root ganglion (DRG) using Anti-ENT1 (SLC29A1) Antibody (#ANT-051), (1:100), followed by goat anti-rabbit-AlexaFluor- 555 secondary antibody. A. ENT1 labeling (red) appears in the cell bodies of the DRG. Note that the nerve fibers are not stained. B. Nuclear staining using DAPI as counterstain (blue). C. Merged images of A+B.
- (C)EQETKLDLISKGEEPR, corresponding to amino acid residues 245-260 of human ENT1 (Accession Q99808).