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Anti-ENT1 (SLC29A1) Antibody

Equilibrative nucleoside transporter 1, Nucleoside transporter es-type

Cat #: ANT-051
Alternative Name Equilibrative nucleoside transporter 1, Nucleoside transporter es-type
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, r
May also work in: m*
Immunogen
  • Peptide (C)EQETKLDLISKGEEPR, corresponding to amino acid residues 245-260 of human ENT1 (Accession Q99808). 3rd intracellular loop.
Accession (Uniprot) Number Q99808
Gene ID 2030
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, rat - 15/16 amino acid residues identical.
RRID AB_2341015.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of rat kidney lysates (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
    Western blot analysis of rat kidney lysates (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
    1,2. Anti-ENT1 (SLC29A1) Antibody (#ANT-051), (1:200).
    3,4. Anti-ENT1 (SLC29A1) Antibody, preincubated with ENT1/SLC29A1 Blocking Peptide (#BLP-NT051).
Immunohistochemistry
  • Expression of ENT1 in rat DRG
    Expression of ENT1 in rat DRG
    Immunohistochemical staining of rat frozen dorsal root ganglion (DRG) using Anti-ENT1 (SLC29A1) Antibody (#ANT-051), (1:100), followed by goat anti-rabbit-AlexaFluor- 555 secondary antibody. A. ENT1 labeling (red) appears in the cell bodies of the DRG. Note that the nerve fibers are not stained. B. Nuclear staining using DAPI as counterstain (blue). C. Merged images of A+B.
References
  1. Molina-Arcas, M. et al. (2009) Curr. Vasc. Pharmacol. 7, 426.
  2. Rose, J.B. and Coe, I.R. (2008) Physiology 23, 41.
  3. Baldwin, S.A. et al. (2004) Pflugers Arch. 447, 735.
  4. Pennycooke, M. et al. (2001) Biochem. Biophys. Res. Commun. 280, 951.
  5. Crawford, C.R. et al. (1998) J. Biol. Chem. 273, 288.
  6. Baldwin, S.A. et al. (2005) J. Biol. Chem. 280, 15880.
  7. Barnes, K. (2006) Circ. Res. 99, 510.
Scientific background

Nucleosides play other important roles beyond their nucleic acid synthesis building block role. For example, they are involved in energy metabolism; they serve as ligands of purinergic receptors and act as influential signaling molecules1. Being hydrophilic, nucleosides cannot simply diffuse across the plasma membrane in order to exert their various functions, but rather need to be physically transported via nucleoside transporters1,2.

Two different transporter families are responsible for transporting nucleosides across the plasma membrane: The Concentrative Nucleoside Transporter proteins (CNT, SLC28 family), which consist of three members, CNT1-3, and act as Na+-dependent symporters1,3, and the Equilibrative Nucleoside Transporter proteins ENT1-4 (ENT, SLC29 family), which mediate Na+-independent facilitated diffusion. ENTs act as bidirectional carriers, responsible for the influx and efflux of substrates1.

Structurally, ENT transporters have eleven transmembrane domains with an intracellular N-terminal and an extracellular C-terminal1.

The best characterized ENT transporters are ENT1 and ENT2, which, although display a broader range of substrate selectivity, have lower affinities for nucleosides compared to concentrative transporters. They are ubiquitously expressed. For example, ENT1 is expressed in erythrocytes, vascular endothelium, the placenta, brain, heart, liver and colon1,4. ENT2 displays more or less the same expression pattern but in addition, is strongly expressed in skeletal muscle1,5. ENT3 is a lysosomal pH-dependent transporter capable of transporting adenine, and ENT4 also transports adenine at acidic pH. They are also broadly expressed with ENT3 displaying high expression in the placenta and ENT4 in the heart6,7.

As mentioned above, nucleosides have a variety of cellular/physiological functions suggesting that transporters responsible for their trafficking may also have functional attributes. Indeed, ENT1 plays a role in proliferation and therefore is responsible for the constitutive trafficking of nucleosides1.

There is no evidence that nucleoside transporters are directly involved in pathophysiologies, but they are clinically significant. For example, nucleoside transporters are responsible for the cellular uptake of a number of nucleoside-derived anticancer drugs1.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Anti-ENT1 (SLC29A1) Antibody (#ANT-051) is a highly specific antibody directed against an epitope of the human equilibrative nucleoside transporter 1. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize ENT1 from rat, mouse, and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Immunohistochemistry citations
  1. Human and rat detrusor sections.
    Silva, I. et al. (2020) Br. J. Pharmacol. 177, 1589.
Immunofluorescence citations
  1. Human and rat detrusor sections.
    Silva, I. et al. (2020) Br. J. Pharmacol. 177, 1589.
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