EphA1 (extracellular) Blocking Peptide (#BLP-ER011) is the original antigen used for immunization during Anti-EphA1 (extracellular) Antibody (#AER-011) generation. The blocking peptide binds and ‘blocks’ Anti-EphA1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:
- Western blot analysis of human Jurkat T cell leukemia cells (lanes 1 and 5), human HeLa cervix adenocarcinoma cells (lanes 2 and 6), human SH-SY5Y neuroblastoma cells (lanes 3 and 7) and human MCF-7 breast adenocarcinoma cells (lanes 4 and 8):
- Expression of EphA1 in rat striatum.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-EphA1 (extracellular) Antibody (#AER-011), (1:300), followed by donkey anti-rabbit-biotin and Streptavidin-Cy3. A. EphA1 immunoreactivity (red) appears in striatal neurons (arrows). B. Pre-incubation of the antibody with EphA1 (extracellular) Blocking Peptide (#BLP-ER011), suppressed staining. Cell nuclei are stained with DAPI (blue).
- (C)KKEPRQLELTWAGSR, corresponding to amino acid residues 457-471 of human EphA1 (Accession P21709).