Ephrin-A1 (extracellular) Blocking Peptide (#BLP-ER031) is the original antigen used for immunization during Anti-Ephrin-A1 (extracellular) Antibody (#AER-031) generation. The blocking peptide binds and ‘blocks’ Anti-Ephrin-A1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse brain membranes (lanes 1 and 3) and human Malme-3M melanoma cell lysate (lanes 2 and 4):
- Western blot analysis of rat heart membranes:
- (C)KITHSPQAHDNPQEK, corresponding to amino acid residues 150-164 of human Ephrin-A1 (Accession P20827).