Anti-Ephrin-A1 (extracellular) Antibody

EPH-Related Receptor Tyrosine Kinase Ligand 1, LERK1, EFNA1
    Cat #: AER-031
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)KITHSPQAHDNPQEK, corresponding to amino acid residues 150-164 of human Ephrin-A1 (Accession P20827). Extracellular.
    Accession (Uniprot) Number P20827
    Gene ID 1942
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, rat - 13/15 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, lci, wb
    May also work in: ifc, ih, ip
    Western blot
    Western blot analysis of mouse brain membranes (lanes 1 and 3) and human Malme-3M melanoma cell lysate (lanes 2 and 4):
    1, 2. Anti-Ephrin-A1 (extracellular) Antibody (#AER-031), (1:200).
    3, 4. Anti- Ephrin-A1 (extracellular) Antibody, preincubated with the control peptide antigen.
    Western blot analysis of rat heart membranes:
    1. Anti- Ephrin-A1 (extracellular) Antibody (#AER-031), (1:400).
    2. Anti- Ephrin-A1 (extracellular) Antibody, preincubated with the control peptide antigen.
    Live cell imaging / Immunocytochemistry
    Expression of Ephrin-A1 in rat PC12 cells
    Immunocytochemical staining of live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells with Anti-Ephrin-A1 (extracellular) Antibody (#AER-031), (1:50), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells. C. Merge of A and B.
    References
    1. Giaginis, C. et al. (2014) BMC Clin. Pathol14, 8.
    2. Goldman-Wohl, D. et al. (2004) Placenta 25, 623.
    3. Pasquale, E.B. (2008) Cell 133, 38.
    4. Yamamoto, H. et al. (2013) Int. J. Oncol. 42, 549.
    Scientific background

    Ephrin-A1, a ligand of type-A Eph receptor tyrosine kinases and the Eph/ephrin system, has been shown to play important roles in the nervous system, tissue patterning and cell adhesion through receptor–ligand interactions and intracellular signaling1. Ephrins can be divided into two subclasses: ephrin A and ephrin B. Ephrin A ligands (ephrin A1-A5) are anchored to the cell surface via a glycosylphosphatidylinositol (GPI) anchor and bind to Eph A receptors (Eph A1-A8), whereas ephrin B ligands (ephrin B1-B3) have transmembrane and cytoplasmic domains and interact with Eph B receptors (Eph B1-B6)2.

    Eph receptors and ephrins are highly expressed in the brain and in the developing nervous system, where they have well-known roles in the establishment of neuronal connectivity by guiding axons to the appropriate targets and regulating the formation of synaptic connections. Ephrins are expressed in numerous regions of the vertebrate embryo including the ectoderm, mesoderm, and endoderm3.

    Ephrin (Eph) receptors are frequently overexpressed in a variety of human malignant tumors, and are associated with tumor growth, invasion, metastasis and angiogenesis. Recent studies using clinical samples showed that ephrin-A1 expression was up-regulated in colorectal cancer and hepatocellular carcinoma and positively correlated with a poor prognostic value4.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 07/11/2018

    Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of human Ephrin-A1. Anti-Ephrin-A1 (extracellular) Antibody (#AER-031) can be used in western blot and live cell imaging applications. The antibody recognizes an extracellular epitope and thus is ideal for detecting Ephrin-A1 in living cells. It has been designed to recognize Ephrin-A1 from human, rat and mouse samples.

    For research purposes only, not for human use