Ephrin-A5 (extracellular) Blocking Peptide (#BLP-ER035) is the original antigen used for immunization during Anti-Ephrin-A5 (extracellular) Antibody (#AER-035) generation. The blocking peptide binds and ‘blocks’ Anti-Ephrin-A5 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysate:
- Western blot analysis of human LNCaP prostate adenocarcinoma cell line lysate:
- Expression of Ephrin-A5 rat hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Ephrin-A5 (extracellular) Antibody (#AER-035), (1:200), followed by goat anti-rabbit-AlexaFluor-488. Ephrin A5 staining (green) in hippocampal dentate gyrus is detected in hilar interneurons (arrows) and in the granule layer (GL). Cell nuclei are stained with DAPI (blue).
- (C)DRVFDVNDKVENSLE, corresponding to amino acid residues 172-186 of human Ephrin-A5 (Accession P52803).