- Peptide (C)DRVFDVNDKVENSLE, corresponding to amino acid residues 172-186 of human Ephrin-A5 (Accession P52803). Extracellular.
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysate:1,2. Anti-Ephrin-A5 (extracellular) Antibody (#AER-035), (1:200).
3,4. Anti-Ephrin-A5 (extracellular) Antibody, preincubated with Ephrin-A5 (extracellular) Blocking Peptide (#BLP-ER035).
- Western blot analysis of human LNCaP prostate adenocarcinoma cell line lysate:1. Anti-Ephrin-A5 (extracellular) Antibody (#AER-035), (1:200).
2. Anti-Ephrin-A5 (extracellular) Antibody, preincubated with Ephrin-A5 (extracellular) Blocking Peptide (#BLP-ER035).
- Expression of Ephrin-A5 rat hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Ephrin-A5 (extracellular) Antibody (#AER-035), (1:200), followed by goat anti-rabbit-AlexaFluor-488. Ephrin A5 staining (green) in hippocampal dentate gyrus is detected in hilar interneurons (arrows) and in the granule layer (GL). Cell nuclei are stained with DAPI (blue).
The Eph family of tyrosine kinase receptor signaling pathway is important for a variety of physiological processes during development including cell morphology, proliferation, migration and axonal guidance, dendritic spine formation and neural plasticity. Eph receptors mediate forward signaling in one cell while ephrin ligands transmit reverse signaling in an adjacent cell1-3.
Eph receptors consist of two large subfamilies: EphA and EphB that bind to either glycophosphatidylinositol (GPI)-anchored ephrin-A ligands or transmembrane ephrin-B ligands respectively. Ephrin ligands are divided into ephrin-A (1 to 5) and ephrin-B (1 to 3)1,2.
Ephrin-A5 is predominantly expressed in the quiescent anterior lens epithelial cells and is highly expressed in different layers of the cortex2,3.
Studies show that mutations in ephrin-A5 are linked to congenital and age-related cataracts in humans and mice. Loss of ephrin-A5 causes abnormal localization of E-cadherin and β-catenin in anterior epithelial cells and epithelial-mesenchymal-transition2.
Species reactivity key:
Anti-Ephrin-A5 (extracellular) Antibody (#AER-035) is a highly specific antibody directed against an epitope of human EFNA5. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize EFNA5 from rat, mouse, and human samples.