Ephrin-B1 (extracellular) Blocking Peptide (#BLP-ER041) is the original antigen used for immunization during Anti-Ephrin-B1 (extracellular) Antibody (#AER-041) generation. The blocking peptide binds and ‘blocks’ Anti-Ephrin-B1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse enriched brain membranes (lanes 1 and 3) and human HT-29 colon adenocarcinoma cells lysate (lanes 2 and 4):
- (C)KHETVNQEEKSGPG, corresponding to amino acid residues 208-221 of human Ephrin-B1 (Accession P98172).