GABA(B) R1 (extracellular) Blocking Peptide (#BLP-GB001) is the original antigen used for immunization during Anti-GABA(B) R1 (extracellular) Antibody (#AGB-001) generation. The blocking peptide binds and ‘blocks’ Anti-GABA(B) R1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes:
- Expression of GABA(B) receptor 1 in mouse hippocampusImmunohistochemical staining of mouse hippocampus frozen sections using Anti-GABA(B) R1 (extracellular) Antibody (#AGB-001), (1:100). A. GABBR1 staining (green) is detected in neurons in the CA3 field and in the dentate granule layer (short arrows), as well as in dendrites of CA3 pyramidal neurons (long arrows). B. Staining with mouse anti-GAP43 antibody (red) sets apart the stratum lacunosum moleculare (SLM). C. Confocal merge suggests the presence of GABBR1 in pyramidal neurons.
- CRGEREVVGPKVRK, corresponding to amino acid residues 66-79 of rat GABBR1 (Accession Q9Z0U4).