Overview
Type: Synthetic peptide
Form: Lyophilized powder
GABA(B) R2 Blocking Peptide (#BLP-GB002) is the original antigen used for immunization during Anti-GABA(B) R2 Antibody (#AGB-002) generation. The blocking peptide binds and ‘blocks’ Anti-GABA(B) R2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membrane:1. Anti-GABA(B) R2 Antibody (#AGB-002), (1:200).
2. Anti-GABA(B) R2 Antibody, preincubated with GABA(B) R2 Blocking Peptide (#BLP-GB002).
Expression of GABA(B) receptor 2 in mouse hippocampusImmunohistochemical staining of mouse hippocampus frozen sections using Anti-GABA(B) R2 Antibody (#AGB-002 ), (1:100). A. GABBR2 staining (green) is detected in layers including the stratum lacunosum moleculare (SLM) and stratum radiatum of the CA3 layer (star). B. Staining with mouse anti-GAP43 antibody (red) sets apart the SLM. C. Confocal merge suggests the presence of GABBR2 in layers, but not in pyramidal neurons.
Properties
Sequence
- CKDPIEDINSPEHIQRRLSL, corresponding to amino acid residues 875-894 of human GABBR2 (Accession O75899).
Accession (Uniprot) Number O75899
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
