GABA(B) R2 Blocking Peptide (#BLP-GB002) is the original antigen used for immunization during Anti-GABA(B) R2 Antibody (#AGB-002) generation. The blocking peptide binds and ‘blocks’ Anti-GABA(B) R2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membrane:
- Expression of GABA(B) receptor 2 in mouse hippocampusImmunohistochemical staining of mouse hippocampus frozen sections using Anti-GABA(B) R2 Antibody (#AGB-002 ), (1:100). A. GABBR2 staining (green) is detected in layers including the stratum lacunosum moleculare (SLM) and stratum radiatum of the CA3 layer (star). B. Staining with mouse anti-GAP43 antibody (red) sets apart the SLM. C. Confocal merge suggests the presence of GABBR2 in layers, but not in pyramidal neurons.
- CKDPIEDINSPEHIQRRLSL, corresponding to amino acid residues 875-894 of human GABBR2 (Accession O75899).