Overview
Type: Synthetic peptide
Form: Lyophilized powder
Gamma-Synuclein Blocking Peptide (#BLP-PZ037) is the original antigen used for immunization during Anti-Gamma-Synuclein Antibody (#APZ-037) generation. The blocking peptide binds and ‘blocks’ Anti-Gamma-Synuclein primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):1, 2. Anti-Gamma-Synuclein Antibody (#APZ-037), (1:200).
3, 4. Anti-Gamma-Synuclein Antibody, preincubated with Gamma-Synuclein Blocking Peptide (#BLP-PZ037).
Expression of Gamma-Synuclein in rat hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Gamma-Synuclein Antibody (#APZ-037), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Gamma-Synuclein immunoreactivity (green) appears in blood vessels (arrows). B. Pre-incubation of the antibody with Gamma-Synuclein Blocking Peptide (#BLP-PZ037), suppresses staining. Cell nuclei are stained with DAPI (blue). G = granule layer.
Properties
Sequence
- (C)RKEDLEPPAQDQE, corresponding to amino acid residues 96 - 108 of rat γ-Synuclein (Accession O76070)
Accession (Uniprot) Number O76070
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Standard Quality Control Of Each Lot Western blot analysis.
