Overview
Type: Synthetic peptide
Form: Lyophilized powder
Ghrelin Receptor/GHSR (extracellular) Blocking Peptide (#BLP-GR031) is the original antigen used for immunization during Anti-Ghrelin Receptor (GHSR) (extracellular) Antibody (#AGR-031) generation. The blocking peptide binds and ‘blocks’ Anti-Ghrelin Receptor/GHSR (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat pancreas (lanes 1 and 4), human SH-SY5Y neuroblastoma (lanes 2 and 5) and mouse MS1 endothelial (lanes 3 and 6) cells line lysates:1-3. Anti-Ghrelin Receptor (GHSR) (extracellular) Antibody (#AGR-031), (1:200).
4-6. Anti-Ghrelin Receptor (GHSR) (extracellular) Antibody, preincubated with Ghrelin Receptor/GHSR (extracellular) Blocking Peptide (#BLP-GR031).
Expression of Ghrelin receptor in rat and mouse hypothalamusImmunohistochemical staining of rat and mouse hypothalamic paraventricular nucleus (PVN) using Anti-Ghrelin Receptor (GHSR) (extracellular) Antibody (#AGR-031), (1:800). A. Ghrelin receptor 1 staining (red) in mouse appears in cells of the PVN (arrows). B. Ghrelin receptor staining in rat is similar. Nuclear staining using DAPI as the counterstain (blue).
Properties
Sequence
- CRATEFAVRSG, corresponding to amino acid residues 197-207 of rat Ghrelin receptor (Accession O08725).
Accession (Uniprot) Number O08725
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
