Anti-Ghrelin Receptor (extracellular) Antibody

Growth hormone secretagogue receptor type 1, GHS-R, GH-releasing peptide receptor, GHRP
    Cat #: AGR-031
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide CRATEFAVRSG, corresponding to amino acid residues 197-207 of rat Ghrelin Receptor (Accession O08725). 2nd extracellular loop.
    Accession (Uniprot) Number O08725
    Gene ID 84022
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human - 10/11 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ih, lci, wb
    May also work in: ifc, ip
    Western blot
    Western blot analysis of rat pancreas (lanes 1 and 4), SH-SY5Y (lanes 2 and 5) and MS1 (lanes 3 and 6) lysates:
    1-3. Anti-Ghrelin Receptor (extracellular) Antibody (#AGR-031), (1:200).
    4-6. Anti-Ghrelin Receptor (extracellular) Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Expression of Ghrelin Receptor in rat and mouse hypothalamus
    Immunohistochemical staining of rat and mouse hypothalamic paraventricular nucleus (PVN) using Anti-Ghrelin Receptor (extracellular) Antibody (#AGR-031), (1:800).  A. Ghrelin receptor 1 staining (red) in mouse appears in cells of the PVN (arrows). B. Ghrelin receptor staining in rat is similar. Nuclear staining using DAPI as the counterstain (blue).
    Live cell imaging / Immunocytochemistry
    Expression of Ghrelin Receptor in human SH-SY5Y cell line
    Immunocytochemical staining of intact living human neuroblastoma (SH-SY5Y) cells. A. Extracellular staining of cells with Anti-Ghrelin Receptor (extracellular) Antibody (#AGR-031), (red), (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. DAPI is used as the counterstain (blue). C. Merged images of A and B.
    References
    1. Sato, T. et al. (2012) J. Biochem. 151, 119.
    2. Kojima, M. et al. (1999) Nature 402, 656.
    3. Albarran-Zeckler, R.G. et al. (2011) Peptides 32, 2229.
    4. Seim, I. et al. (2011) Mol. Cell. Endo. 340, 3.
    5. Takahashi, K. et al. (2006) Cancer Res. 66, 9408.
    Scientific background

    Ghrelin is a 28 amino acid peptide, acylated on a serine residue which is essential for its biological activity1,2. In rodents, an additional form of the peptide could be purified. The peptide is released from the stomach and exerts its effect on the ghrelin receptor (GHS-R). GHS-R is a member of the G-protein coupled receptor (GPCR) superfamily. Like all members, the receptor has seven transmembrane spanning domains and upon activation it stimulates phospholipase C, thereby increasing IP3 and intracellular Ca2+ levels1,3.

    GHS-R is expressed in the hypothalamus, pituitary and hippocampus1,3.

    Some of the actions of ghrelin include the stimulation of growth hormone release, food intake, fat accumulation, regulation of memory and learning, activation of reward-related pathways and neuroprotection to name a few1,3.

    GHS-R 1b is a truncated form of the full length receptor which is also expressed. Although its exact functions are unknown, it is believed that it may be involved in downregulating signaling from the full length receptor4. In addition, it is overexpressed in lung cancer where it dimerizes with neurotensin receptor4,5.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 02/12/2018

    Anti-Ghrelin Receptor (extracellular) Antibody (#AGR-031) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, live cell imaging, and immunocytochemistry applications. It has been designed to recognize ghrelin receptor from human, mouse, and rat samples.

    For research purposes only, not for human use
    Citations
    Related Products