GIRK3/Kir3.3 Blocking Peptide (#BLP-PC038) is the original antigen used for immunization during Anti-GIRK3 (Kir3.3) Antibody (#APC-038) generation. The blocking peptide binds and ‘blocks’ Anti-GIRK3/Kir3.3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes:
- Rat brain sections.
- Expression of GIRK3 in mouse deep cerebellar nuclei (DCN).Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GIRK3 (Kir3.3) Antibody (#APC-038), (1:400), followed by goat anti-rabbit-Alexa-488. A. GIRK3 immunoreactivity (green) appears in DCN cells (arrows). B. Pre-incubation of the antibody with GIRK3 (Kir3.3) Blocking Peptide (#BLP-PC038), suppressed staining. Cell nuclei are stained with DAPI (blue).
- (C)RLDAHLYWSIPSRLDEKV, corresponding to residues 344-361 of rat KCNJ9 (Accession Q63511).