Overview
Type: Synthetic peptide
Form: Lyophilized powder
GIRK3/Kir3.3 Blocking Peptide (#BLP-PC038) is the original antigen used for immunization during Anti-GIRK3 (Kir3.3) Antibody (#APC-038) generation. The blocking peptide binds and ‘blocks’ Anti-GIRK3/Kir3.3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-GIRK3 (Kir3.3) Antibody (#APC-038), (1:200).
2. Anti-GIRK3 (Kir3.3) Antibody, preincubated with GIRK3/Kir3.3 Blocking Peptide (#BLP-PC038).- Rat brain sections.
Expression of GIRK3 in mouse deep cerebellar nuclei (DCN).Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GIRK3 (Kir3.3) Antibody (#APC-038), (1:400), followed by goat anti-rabbit-Alexa-488. A. GIRK3 immunoreactivity (green) appears in DCN cells (arrows). B. Pre-incubation of the antibody with GIRK3 (Kir3.3) Blocking Peptide (#BLP-PC038), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)RLDAHLYWSIPSRLDEKV, corresponding to residues 344-361 of rat KCNJ9 (Accession Q63511).
Accession (Uniprot) Number Q63511
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 3 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
