Overview
Type: Synthetic peptide
Form: Lyophilized powder
GLP1R (extracellular) Blocking Peptide (#BLP-GR021) is the original antigen used for immunization during Anti-GLP1R (extracellular) Antibody (#AGR-021) generation. The blocking peptide binds and ‘blocks’ Anti-GLP1R (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat pancreas lysate (lanes 1 and 5), mouse preadipocyte 3T3-L1 lysate (lanes 2 and 6), rat pancreatic islet cell line RIN-5F lysate (lanes 3 and 7) and human pancreatic carcinoma PANC-1 lysate (lanes 4 and 8):1-4. Anti-GLP1R (extracellular) Antibody (#AGR-021), (1:200).
5-8. Anti-GLP1R (extracellular) Antibody, preincubated with GLP1R (extracellular) Blocking Peptide (#BLP-GR021).
Expression of Glucagon-like peptide 1 receptor in rat pancreasImmunohistochemical staining of rat pancreas paraffin embedded sections using Anti-GLP1R (extracellular) Antibody (#AGR-021), (1:100). Staining (brown color) is present in endocrine cells of the Isles of Langerhans. Hematoxilin is used as the counterstain.
Expression of Glucagon-like peptide 1 receptor in rat hypothalamusImmunohistochemical staining of rat hypothalamus with Anti-GLP1R (extracellular) Antibody (#AGR-021), (1:200). Glucagon-like peptide 1 receptor (green) appears in the lateral hypothalamic region axonal profiles (vertical arrow) and nerve cell profiles (horizontal arrows). DAPI is used as the counterstain (blue).
Properties
Sequence
- (C)KYLYEDEG(S)WTRNS, corresponding to amino acid residues 288-301 of rat GLP1R (Accession P32301).
Accession (Uniprot) Number P32301
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
