GLP1R (extracellular) Blocking Peptide (#BLP-GR021) is the original antigen used for immunization during Anti-GLP1R (extracellular) Antibody (#AGR-021) generation. The blocking peptide binds and ‘blocks’ Anti-GLP1R (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat pancreas lysate (lanes 1 and 5), mouse preadipocyte 3T3-L1 lysate (lanes 2 and 6), rat pancreatic islet cell line RIN-5F lysate (lanes 3 and 7) and human pancreatic carcinoma PANC-1 lysate (lanes 4 and 8):
- Expression of Glucagon-like peptide 1 receptor in rat pancreasImmunohistochemical staining of rat pancreas paraffin embedded sections using Anti-GLP1R (extracellular) Antibody (#AGR-021), (1:100). Staining (brown color) is present in endocrine cells of the Isles of Langerhans. Hematoxilin is used as the counterstain.
- Expression of Glucagon-like peptide 1 receptor in rat hypothalamusImmunohistochemical staining of rat hypothalamus with Anti-GLP1R (extracellular) Antibody (#AGR-021), (1:200). Glucagon-like peptide 1 receptor (green) appears in the lateral hypothalamic region axonal profiles (vertical arrow) and nerve cell profiles (horizontal arrows). DAPI is used as the counterstain (blue).
- (C)KYLYEDEG(S)WTRNS, corresponding to amino acid residues 288-301 of rat GLP1R (Accession P32301).