GluR4/GluA4 (extracellular) Blocking Peptide (#BLP-GC019) is the original antigen used for immunization during Anti-GluR4 (GluA4) (extracellular) Antibody (#AGC-019) generation. The blocking peptide binds and ‘blocks’ Anti-GluR4/GluA4 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
- Expression of GRIA4 (GluR4) in rat DRGImmunohistochemical staining of rat frozen dorsal root ganglion (DRG) sections using Anti-GluR4 (GluA4) (extracellular) Antibody (#AGC-019), (1:100). GRIA4 is expressed in all the DRG neurons (red). Hoechst 33342 is used as the counterstain (blue).
- (C)KLMDRWKKLDQRE, corresponding to amino acid residues 274-286 of rat GluR4 (Accession P19493).