Overview
Cat #: BLP-GC019
Type: Synthetic peptide
Form: Lyophilized powder
GluR4/GluA4 (extracellular) Blocking Peptide (#BLP-GC019) is the original antigen used for immunization during Anti-GluR4 (GluA4) (extracellular) Antibody (#AGC-019) generation. The blocking peptide binds and ‘blocks’ Anti-GluR4/GluA4 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1,2. Anti-GluR4 (GluA4) (extracellular) Antibody (#AGC-019), (1:200).
3,4. Anti-GluR4 (GluA4) (extracellular) Antibody, preincubated with GluR4/GluA4 (extracellular) Blocking Peptide (#BLP-GC019). - Expression of GRIA4 (GluR4) in rat DRGImmunohistochemical staining of rat frozen dorsal root ganglion (DRG) sections using Anti-GluR4 (GluA4) (extracellular) Antibody (#AGC-019), (1:100). GRIA4 is expressed in all the DRG neurons (red). Hoechst 33342 is used as the counterstain (blue).
Properties
Sequence
Accession (Uniprot) Number P19493
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.