Anti-GluR4 (GluA4) (extracellular) Antibody

AMPA Receptor 4, Glutamate receptor 4, Ionotropic glutamate receptor 4, AMPA-selective glutamate receptor 4, GRIA4, GluR-D
Cat #: AGC-019
Alternative Name AMPA Receptor 4, Glutamate receptor 4, Ionotropic glutamate receptor 4, AMPA-selective glutamate receptor 4, GRIA4, GluR-D
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
      • Peptide (C)KLMDRWKKLDQRE, corresponding to amino acid residues 274-286 of rat GluR4 (Accession P19493). Extracellular, N-terminus.
    Accession (Uniprot) Number P19493
    Gene ID 29629
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human - identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.6 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
    May also work in: ic, ifc, ip, lci
      • Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
        1,2. Anti-GluR4 (GluA4) (extracellular) Antibody (#AGC-019), (1:200).
        3,4. Anti-GluR4 (GluA4) (extracellular) Antibody, preincubated with the control peptide antigen.
      • Expression of GRIA4 (GluR4) in rat DRG
        Immunohistochemical staining of rat frozen dorsal root ganglion (DRG) sections using Anti-GluR4 (GluA4) (extracellular) Antibody (#AGC-019), (1:100). GRIA4 is expressed in all the DRG neurons (red). Hoechst 33342 is used as the counterstain (blue).
      • Glutamate is the major excitatory neurotransmitter and modulates its effects via ionotropic and metabotropic receptors which are different in their molecular, biochemical, pharmacological and physiological properties1.

        The ionotropic ligand-gated ion channel glutamate receptors are classified into three major subtypes, AMPA, kainate and NMDA receptors according to their selective agonist.

        AMPA receptors consist of four closely related genes with about 70% homology that encode the four subunits GluR1-4. Their expression is known to be developmentally regulated2 and are also known to undergo alternative splicing. In fact, all four receptors have two splice variants, termed flip and flop, in an extracellular region adjacent to the M4 transmembrane region3. These splice variants are also developmentally regulated such that flip is expressed before birth and remains highly expressed throughout adulthood whereas flop expression is postnatal and reaches flip’s level in adulthood4.

        In addition to their expression regulation, flip and flop also differ in their activation/inactivation kinetics. Each AMPA receptor contains 3 membrane spanning domains (M1, M3 and M4), while the fourth hydrophobic domain (M2) is a reentering cytoplasmic loop that forms part of the channels pore1. Different stoichiometries of these receptors form tetrameric structures to eventually form functional receptors5.

        AMPA receptors are highly expressed in the brain. However, the GluR4 subunit is present in lower amounts in the CNS, except in the reticular thalamic neuclei and the cerebellum1.

        The primary depolarization in glutamate neurotransmission is mediated by AMPA receptors, which are also key players in synaptic plasticity, learning and memory formation. The cellular distribution and post-translational modifications like phosphorylation are important for the activity of AMPA receptors and are thought to play a role in the long lasting and activity-dependent changes in synaptic strength6-9.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 18/07/2019

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat ionotropic glutamate receptor 4. Anti-GluR4 (GluA4) (extracellular) Antibody (#AGC-019) can be used in western blot and immunohistochemistry applications. It has been designed to recognize GluR4 from rat, mouse, and human samples.

    For research purposes only, not for human use