GLUT3 (extracellular) Blocking Peptide (#BLP-GT023) is the original antigen used for immunization during Anti-GLUT3 (extracellular) Antibody (#AGT-023) generation. The blocking peptide binds and ‘blocks’ Anti-GLUT3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
- Expression of Glucose transporter 3 in mouse hippocampus and cerebellumImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GLUT3 (extracellular) Antibody (#AGT-023), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. GLUT3 staining (green) in mouse hippocampal dentate gyrus, is detected in interneurons (arrows pointing up) in the Hilus and granule layer (G) (arrow pointing down). B. Staining in mouse cerebellum, is seen in purkinje cells (vertical arrows) and dendrites (horizontal arrows) in the molecular layer (Mol). Cell nuclei were stained with DAPI (blue).
- (C)KDFLNYTLEERLED, corresponding to amino acid residues 39-52 of rat Glucose Transporter 3 (Accession Q07647).