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Anti-GLUT3 (extracellular) Antibody

Glucose transporter 3, Facilitated glucose transporter member 3, SLC2A3

Cat #: AGT-023
Alternative Name Glucose transporter 3, Facilitated glucose transporter member 3, SLC2A3
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
Immunogen
  • Peptide (C)KDFLNYTLEERLED, corresponding to amino acid residues 39-52 of rat Glucose Transporter 3 (Accession Q07647). 1st extracellular loop.
Accession (Uniprot) Number Q07647
Gene ID 100909595
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – identical.
RRID AB_2756644.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity Will not recognize human GLUT3.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ifc, ih, wb
May also work in: ic*, ip*, lci*
Western blot
  • Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    1, 2. Anti-GLUT3 (extracellular) Antibody (#AGT-023), (1:400).
    3, 4. Anti-GLUT3 (extracellular) Antibody, preincubated with GLUT3 (extracellular) Blocking Peptide (#BLP-GT023).
Immunohistochemistry
  • Expression of Glucose transporter 3 in mouse hippocampus and cerebellum
    Expression of Glucose transporter 3 in mouse hippocampus and cerebellum
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GLUT3 (extracellular) Antibody (#AGT-023), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. GLUT3 staining (green) in mouse hippocampal dentate gyrus, is detected in interneurons (arrows pointing up) in the Hilus and granule layer (G) (arrow pointing down). B. Staining in mouse cerebellum, is seen in purkinje cells (vertical arrows) and dendrites (horizontal arrows) in the molecular layer (Mol). Cell nuclei were stained with DAPI (blue).
Indirect flow cytometry
  • Cell surface detection of Glucose transporter 3 in live intact mouse J774 macrophage cell line:
    Cell surface detection of Glucose transporter 3 in live intact mouse J774 macrophage cell line:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-GLUT3 (extracellular) Antibody (#AGT-023), (5 µg) + goat-anti-rabbit-FITC.
References
  1. Lacombe, V.A. (2014) ISRN Vet. Sci. 2014, 409547.
  2. Simpson, I.A. et al. (2008) Am J. Physiol. 295, E242.
  3. Zheng, C. et al. (2016) Oncol. Lett. 12, 125.
Scientific background

The GLUT protein family is encoded by the SLC2 genes and are a member of the major facilitator superfamily of membrane transporters. GLUTs are recognized as key regulators of whole-body glucose homeostasis, and are responsible for the uptake of carbohydrate-derived glucose by cells. There are 14 GLUT isoforms divided into 3 different group classes based on sequence similarity and structural and functional characteristics1,2.

GLUT3 is a low-Km protein responsible for glucose uptake into neurons. It is predominantly expressed in neurons and is commonly called “neuronal glucose transporter". The protein contains 12 membrane-spanning domains, an N-linked glycosylation site in loop 1 responsible for high-affinity transport of glucose, and intracellular NH2- and COOH- termini. In addition, there are several conserved residues and motifs designated “sugar transporter signatures”1,2.

GLUT3 levels are high in some malignant glioma cells compared to normal glial cells and therefore may be used as a therapeutic target in some cancer treatment3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 21/12/2020

Anti-GLUT3 (extracellular) Antibody (#AGT-023) is a highly specific antibody directed against an epitope of the rat Glucose Transporter 3 protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. The antibody has been designed to recognize GLUT3 from rat and mouse samples. It will not recognize GLUT3 from human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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