GLUT4 Blocking Peptide (#BLP-GT024) is the original antigen used for immunization during Anti-GLUT4 Antibody (#AGT-024) generation. The blocking peptide binds and ‘blocks’ Anti-GLUT4 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) heart membranes:
- Expression of Glucose Transporter 4 in rat olfactory bulbImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-GLUT4 Antibody (#AGT-024), (1:400), followed by goat-anti-rabbit-AlexaFluor-488. GLUT4 staining (green) appears in some glomeruli (“G” and horizontal arrows), but not in others (vertical arrow). Cell nuclei are stained with DAPI (blue).
- (C)KPSTELEYLGPDE, corresponding to amino acid residues 495-507 of human Glucose Transporter 4 (Accession P14672).