GLUT5/SLC2A5 Blocking Peptide (#BLP-GT025) is the original antigen used for immunization during Anti-GLUT5 (SLC2A5) Antibody (#AGT-025) generation. The blocking peptide binds and ‘blocks’ Anti-GLUT5/SLC2A5 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
- Western blot analysis of rat small intestine lysate:
- Western blot analysis of human MCF-7 breast adenocarcinoma cell lysates (lanes 1 and 3) and human Caco-2 colon adenocarcinoma cell lysates (lanes 2 and 4):
- Expression of Glucose Transporter 5 in mouse hippocampus and cerebellumImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GLUT5 (SLC2A5) Antibody (#AGT-025), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. A. GLUT5 staining (green) in hippocampal CA3 region, is detected in cells of the pyramidal layer (P) and in their apical dendrites (arrows). B. GLUT5 staining (green) in cerebellum appears in cells of the Purkinje layer (P) and in their apical dendrites (arrows). Cell nuclei are stained with DAPI (blue).
- (C)KNKVSDVYPEKEEK, corresponding to amino acid residues 477 - 490 of rat Glucose Transporter 5 (Accession P43427).