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Anti-GLUT5 (SLC2A5) Antibody

Glucose transporter 5, Facilitated glucose transporter member 5, Fructose transporter

Cat #: AGT-025
Alternative Name Glucose transporter 5, Facilitated glucose transporter member 5, Fructose transporter
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)KNKVSDVYPEKEEK, corresponding to amino acid residues 477 - 490 of rat Glucose Transporter 5 (Accession P43427). Intracellular, C-terminus.
Accession (Uniprot) Number P43427
Gene ID 65197
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – 12/14 amino acid residues identical; human – 11/14 amino acid residues identical.
RRID AB_2756647.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
    Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
    1,2. Anti-GLUT5 (SLC2A5) Antibody (#AGT-025), (1:400).
    3,4. Anti-GLUT5 (SLC2A5) Antibody, preincubated with GLUT5/SLC2A5 Blocking Peptide (#BLP-GT025).
  • Western blot analysis of rat small intestine lysate:
    Western blot analysis of rat small intestine lysate:
    1. Anti-GLUT5 (SLC2A5) Antibody (#AGT-025), (1:200).
    2. Anti-GLUT5 (SLC2A5) Antibody, preincubated with GLUT5/SLC2A5 Blocking Peptide (#BLP-GT025).
  • Western blot analysis of human MCF-7 breast adenocarcinoma cell lysates (lanes 1 and 3) and human Caco-2 colon adenocarcinoma cell lysates (lanes 2 and 4):
    Western blot analysis of human MCF-7 breast adenocarcinoma cell lysates (lanes 1 and 3) and human Caco-2 colon adenocarcinoma cell lysates (lanes 2 and 4):
    1,2. Anti-GLUT5 (SLC2A5) Antibody (#AGT-025), (1:200).
    3,4. Anti-GLUT5 (SLC2A5) Antibody, preincubated with GLUT5/SLC2A5 Blocking Peptide (#BLP-GT025).
Immunohistochemistry
  • Expression of Glucose Transporter 5 in mouse hippocampus and cerebellum
    Expression of Glucose Transporter 5 in mouse hippocampus and cerebellum
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GLUT5 (SLC2A5) Antibody (#AGT-025), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. A. GLUT5 staining (green) in hippocampal CA3 region, is detected in cells of the pyramidal layer (P) and in their apical dendrites (arrows). B. GLUT5 staining (green) in cerebellum appears in cells of the Purkinje layer (P) and in their apical dendrites (arrows). Cell nuclei are stained with DAPI (blue).
References
  1. Lacombe, V.A. (2014) ISRN Vet. Sci. 2014, 409547.
  2. Nomura, N. et al. (2014) Nature 526, 397.
Scientific background

The GLUT protein family belongs to the solute carrier 2 family (SLC2) and is a member of the major facilitator superfamily of membrane transporters. The GLUT proteins have a fold consisting of two symmetrical six transmembrane helix (TM) bundle. In addition, the proteins contain 12 membrane-spanning domains, an N-linked glycosylation site, and intracellular NH2 and COOH termini, and a several conserved residues and motifs designated “sugar transporter signatures”1,2.

Fourteen GLUT isoforms have been identified and are divided into 3 different group classes based on sequence similarity and structural and functional characteristics. GLUT 5,7,9 and 11 belong to class ll group1.

The GLUT transporters are uniporters, which facilitate the diffusion of monosaccharides like glucose and fructose across the cell membrane in a concentration-dependent manner. GLUT5 is specific to fructose and is primarily expressed in the small intestine, but lower levels were also detected in the brain, adipose tissue, kidney, testes and skeletal muscle. Several metabolic disorders and cancers are linked to increased GLUT5 expression2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 21/12/2020

Anti-GLUT5 (SLC2A5) Antibody (#AGT-025) is a highly specific antibody directed against an epitope of the rat Glucose Transporter 5 protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GLUT5 from human, rat, and mouse samples.

For research purposes only, not for human use
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