Anti-GLUT5 (SLC2A5) Antibody

Glucose transporter 5, Facilitated glucose transporter member 5, Fructose transporter
    Cat #: AGT-025
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)KNKVSDVYPEKEEK, corresponding to amino acid residues 477 - 490 of rat Glucose Transporter 5 (Accession P43427). Intracellular, C-terminus.
    Accession (Uniprot) Number P43427
    Gene ID 65197
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse – 12/14 amino acid residues identical; human – 11/14 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
    May also work in: ic, ifc, ip
    Western blot
    Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
    1,2. Anti-GLUT5 (SLC2A5) Antibody (#AGT-025), (1:400).
    3,4. Anti-GLUT5 (SLC2A5) Antibody, preincubated with the control peptide antigen.
    Western blot analysis of rat small intestine lysate:
    1. Anti-GLUT5 (SLC2A5) Antibody (#AGT-025), (1:200).
    2. Anti-GLUT5 (SLC2A5) Antibody, preincubated with the control peptide antigen.
    Western blot analysis of human MCF-7 breast adenocarcinoma cell lysates (lanes 1 and 3) and human Caco-2 colon adenocarcinoma cell lysates (lanes 2 and 4):
    1,2. Anti-GLUT5 (SLC2A5) Antibody (#AGT-025), (1:200).
    3,4. Anti-GLUT5 (SLC2A5) Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Expression of Glucose Transporter 5 in mouse hippocampus and cerebellum
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GLUT5 (SLC2A5) Antibody (#AGT-025), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. A. GLUT5 staining (green) in hippocampal CA3 region, is detected in cells of the pyramidal layer (P) and in their apical dendrites (arrows). B. GLUT5 staining (green) in cerebellum appears in cells of the Purkinje layer (P) and in their apical dendrites (arrows). Cell nuclei are stained with DAPI (blue).
    References
    1. Lacombe, V.A. (2014) ISRN Vet. Sci. 2014, 409547.
    2. Nomura, N. et al. (2014) Nature 526, 397.
    Scientific background

    The GLUT protein family belongs to the solute carrier 2 family (SLC2) and is a member of the major facilitator superfamily of membrane transporters. The GLUT proteins have a fold consisting of two symmetrical six transmembrane helix (TM) bundle. In addition, the proteins contain 12 membrane-spanning domains, an N-linked glycosylation site, and intracellular NH2 and COOH termini, and a several conserved residues and motifs designated “sugar transporter signatures”1,2.

    Fourteen GLUT isoforms have been identified and are divided into 3 different group classes based on sequence similarity and structural and functional characteristics. GLUT 5,7,9 and 11 belong to class ll group1.

    The GLUT transporters are uniporters, which facilitate the diffusion of monosaccharides like glucose and fructose across the cell membrane in a concentration-dependent manner. GLUT5 is specific to fructose and is primarily expressed in the small intestine, but lower levels were also detected in the brain, adipose tissue, kidney, testes and skeletal muscle. Several metabolic disorders and cancers are linked to increased GLUT5 expression2.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 22/11/2018

    Anti-GLUT5 (SLC2A5) Antibody (#AGT-025) is a highly specific antibody directed against an epitope of the rat Glucose Transporter 5 protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GLUT5 from human, rat, and mouse samples.

    For research purposes only, not for human use