GPER/GPR30 Blocking Peptide (#BLP-ER050) is the original antigen used for immunization during Anti-GPER (GPR30) Antibody (#AER-050) generation. The blocking peptide binds and ‘blocks’ Anti-GPER/GPR30 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat lung (lanes 3 and 6) membranes:
- Expression of GPER in rat dentate gyrusImmunohistochemical staining of rat hippocampal dentate gyrus using Anti-GPER (GPR30) Antibody (#AER-050). A. GPER staining (green) appears in cells and fibers of the hilus (arrows) and granule layer (G) B. Parvalbumin (red) is detected in cells in the DG hilus (arrows). C. Nuclei staining using DAPI as the counterstain (blue). D. Merge image of panels A and B reveals colocalization in some cells (arrows).
- (C)ETFRDKLRLYVAQK, corresponding to amino acid residues 329-342 of rat GPER (Accession O08878).