Overview
Type: Synthetic peptide
Form: Lyophilized powder
GPER/GPR30 Blocking Peptide (#BLP-ER050) is the original antigen used for immunization during Anti-GPER (GPR30) Antibody (#AER-050) generation. The blocking peptide binds and ‘blocks’ Anti-GPER/GPR30 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat lung (lanes 3 and 6) membranes:1-3. Anti-GPER (GPR30) Antibody (#AER-050), (1:200).
4-6. Anti-GPER (GPR30) Antibody, preincubated with GPER/GPR30 Blocking Peptide (#BLP-ER050).
Expression of GPER in rat dentate gyrusImmunohistochemical staining of rat hippocampal dentate gyrus using Anti-GPER (GPR30) Antibody (#AER-050). A. GPER staining (green) appears in cells and fibers of the hilus (arrows) and granule layer (G) B. Parvalbumin (red) is detected in cells in the DG hilus (arrows). C. Nuclei staining using DAPI as the counterstain (blue). D. Merge image of panels A and B reveals colocalization in some cells (arrows).
Properties
Sequence
- (C)ETFRDKLRLYVAQK, corresponding to amino acid residues 329-342 of rat GPER (Accession O08878).
Accession (Uniprot) Number O08878
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
