GPR17 Blocking Peptide (#BLP-GR048) is the original antigen used for immunization during Anti-GPR17 Antibody (#AGR-048) generation. The blocking peptide binds and ‘blocks’ Anti-GPR17 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
- Expression of GPR17 in rat parietal cortexImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-GPR17 Antibody (#AGR-048), (1:300), followed by donkey-anti-rabbit-biotin and streptavidin-Cy3. GPR17 immunoreactivity (red) appears in cortical cells (arrows). Cell nuclei are stained with DAPI (blue).
- (C)RQGPRIEKHLKNK, corresponding to amino acid residues 220-232 of rat GPR17 (Accession Q09QM4).