GPR40/FFAR1 (extracellular) Blocking Peptide (#BLP-FR011) is the original antigen used for immunization during Anti-GPR40/FFAR1 (extracellular) Antibody (#AFR-011) generation. The blocking peptide binds and ‘blocks’ Anti-GPR40/FFAR1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat pancreas lysate:
- Expression of GPR40/FFAR1 in rat brainImmunohistochemical staining of rat brain sections using Anti-GPR40/FFAR1 (extracellular) Antibody (#AFR-011). A. GPR40 immunoreactivity (green) in the cerebellum appears in the molecular layer (MOL) and in Purkinje cells (arrows). Nuclei are demonstrated using DAPI as the counterstain (blue).
- (C)NVASFINPDLEGSWRK corresponding to amino acid residues 244-259 of rat FFAR1 (Accession Q8K3T4).