GRID1 (extracellular) Blocking Peptide (#BLP-GC038) is the original antigen used for immunization during Anti-GRID1 (extracellular) Antibody (#AGC-038) generation. The blocking peptide binds and ‘blocks’ Anti-GRID1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of human CCF-STTG1 astrocytoma cell lysate (lanes 1 and 4), mouse brain lysate (lanes 2 and 5) and rat brain lysate (lines 3 and 6):
- Expression of Glutamate receptor δ1 in rat cortex and medial septumImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-GRID1 (extracellular) Antibody (#AGC-038), (1:400). A. Staining in cortex. B. Staining in medial septum. In both regions, GluD1 expression (red) is detected in neurons (arrows). DAPI is used as the counterstain (blue).
- (C)KDMRKLATWDSEK, corresponding to amino acid residues 407-419 of rat GluD1 (Accession Q62640).