Anti-Glutamate Receptor δ1 (GluD1) (extracellular) Antibody

Ionotropic Glutamate receptor Delta 1, GRID1
    Cat #: AGC-038
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)KDMRKLATWDSEK, corresponding to amino acid residues 407-419 of rat GluD1 (Accession Q62640). Extracellular, N-terminus.
    Accession (Uniprot) Number Q62640
    Gene ID 79219
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human, mouse - identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ih, lci, wb
    May also work in: ifc, ip
    Western blot
    Western blot analysis of human CCF-STTG1 astrocytoma cell lysate (lanes 1 and 4), mouse brain lysate (lanes 2 and 5) and rat brain lysate (lines 3 and 6):
    1-3. Anti-Glutamate Receptor δ1 (GluD1) (extracellular) Antibody (#AGC-038), (1:200).
    4-6. Anti-Glutamate Receptor δ1 (GluD1) (extracellular) Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Expression of Glutamate Receptor δ1 in rat cortex and medial septum
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Glutamate Receptor δ1 (GluD1) (extracellular) Antibody (#AGC-038), (1:400). A. Staining in cortex. B.  Staining in medial septum. In both regions, GluD1 expression (red) is detected in neurons (arrows). DAPI is used as the counterstain (blue).
    Live cell imaging / Immunocytochemistry
    Expression of GluD1 in rat PC12 cells
    Immunocytochemical staining of live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells with Anti-Glutamate Receptor δ1 (GluD1) (extracellular) Antibody (#AGC-038), (1:50), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells. C. Merge of A and B.
    References
    1. Schmid, S. M. et al. (2009) Proc. Natl. Acad. Sci. U.S.A. 106, 10320.
    2. Traynelis, S.F. et al. (2010) Pharmacol. Rev. 62, 405.
    3. Lomeli, H. et al. (1993) FEBS Lett. 315, 318.
    4. Safieddine, S. and Wenthold, R.J. (1997) J. Neurosci. 17, 7523.
    5. Gao, J. et al. (2007) Mol. Cell Biol. 27, 4500.
    6. Treutlein, J. et al. (2009) Schizophr. Res. 111, 123.
    7. Smith, M. et al.   (2009) Ann N Y Acad Sci 1151, 102.
    8. Balciuniene, J. et al. (2007) Am. J. Hum. Genet. 80, 938.
    Scientific background

    Excitatory neurotransmission in the vertebrate central nervous system is mainly mediated by ionotropic glutamate receptors (iGluRs). Molecular cloning identified 18 mammalian iGluR subunits, of which only 16 sort into the traditional pharmacological subfamilies of AMPAkainate (KA), and N-methyl-D-aspartate (NMDA) receptors. The 2 remaining subunits were termed “orphan” receptors, “glutamate-like” receptors, “nonionotropic” receptors, or, most commonly, delta receptors1.

    Ionotropic glutamate receptors are integral membrane proteins composed of four large subunits that form a central ion channel pore. Sequence similarity among all known glutamate receptor subunits, including the δ receptors, suggests they share a similar architecture. Glutamate receptor subunits are modular structures that contain four discrete semiautonomous domains: the extracellular amino-terminal domain (ATD), the extracellular ligand-binding domain (LBD), the transmembrane domain (TMD), and an intracellular carboxyl-terminal domain (CTD)2

    The delta family of ionotropic glutamate receptors (iGluRs) consists of the glutamate δ1 (GluD1) and glutamate δ2 (GluD2) receptors3. GluD1 is highly expressed in the inner hair cells of the organ of Corti4, diffusely expressed throughout the forebrain during development with high levels in the hippocampus during adulthood3. Deletion of GluD1 leads to a deficit in high frequency hearing in mice5.  Genetic association studies have established the GRID1 gene, which codes for GluD1, is a strong candidate gene for schizophrenia, bipolar disorder, and major depressive disorder6. Copy number variation studies have also implicated GRID1 in autism spectrum disorder (ASD)7. In addition, GRID1 gene is localized to the 10q22–q23 genomic region which is a site for recurrent deletions associated with cognitive and behavioral abnormalities8.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 21/11/2018

    Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat Glutamate Receptor δ1. Anti-Glutamate Receptor δ1 (GluD1) (extracellular) Antibody (#AGC-038) can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize GluD1 from rat, mouse, and human samples.

    For research purposes only, not for human use