GRIK1/GluK1 (extracellular) Blocking Peptide (#BLP-GC008) is the original antigen used for immunization during Anti-GRIK1 (GluK1) (extracellular) Antibody (#AGC-008) generation. The blocking peptide binds and ‘blocks’ Anti-GRIK1/GluK1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes:
- Expression of GluK1 in rat cerebellumImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-GRIK1 (GluK1) (extracellular) Antibody (#AGC-008), (1:400), followed by donkey anti rabbit-Cy3 antibody. GluK1 staining (red) appears in the granule layer and in the baskets under Purkinje cells (arrows). Nuclei were labeled with DAPI and appear in blue.
- (C)KASGEVSKHLYKVWKK, corresponding to amino acid residues 402-417 of rat kainate receptor GluK1 (Accession P22756).