GRIK4/KA1 (extracellular) Blocking Peptide (#BLP-GC041) is the original antigen used for immunization during Anti-GRIK4 (KA1) (extracellular) Antibody (#AGC-041) generation. The blocking peptide binds and ‘blocks’ Anti-GRIK4/KA1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:
- Expression of kainate receptor GluK4 in rat cerebellar deep nucleiImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-GRIK4 (KA1) (extracellular) Antibody (#AGC-041), (1:100). A. GluK4 staining (red) appears in neurons (arrows) of deep cerebellar nuclei. B. Cell nuclei in the same section are visualized with DAPI (blue). C. Merge of the two images.
- (C)KNRINRAPERLGK, corresponding to amino acid residues 49-61 of rat kainate receptor GluK4 (Accession Q01812).