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Guinea Pig Anti-GFAP Antibody

Glial fibrillary acidic protein

Cat #: AGP-307
Alternative Name Glial fibrillary acidic protein
Lyophilized Powder yes
Type: Polyclonal
Host: Guinea Pig
Reactivity: m, r
Immunogen
  • Peptide (C)RHLGTIPRLSLSR, corresponding to amino acid residues 27-39 of rat Glial fibrillary acidic protein (Accession P47819). Intracellular, cytoplasm.
Accession (Uniprot) Number P47819
Gene ID 24387
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – 10/13 amino acid residues identical. The antibody will not recognize GFAP from human samples.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Guinea pig total IgG
Specificity The antibody will not recognize GFAP from human samples.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of rat brain synaptosome lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):
    Western blot analysis of rat brain synaptosome lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):
    1-2. Guinea Pig Anti-GFAP Antibody (#AGP-307), (1:500).
    3-4. Guinea Pig Anti-GFAP Antibody, preincubated with GFAP Blocking Peptide (BLP-FP001).
Immunohistochemistry
  • Expression of GFAP in rat parietal cortex.
    Expression of GFAP in rat parietal cortex.
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Guinea Pig Anti-GFAP Antibody (AGP-307). Sections were incubated with guinea pig anti GFAP (#AGP-307), (1:1200), followed by goat anti guinea pig conjugated with Alexa 594 (red). A, GFAP immunoreactivity appears in cortical astrocytes in layers 1-2 (arrows). B, Pre-incubation of the antibody with GFAP blocking peptide (BLP-FP001), suppressed staining. Cell nuclei are stained with DAPI (blue).
References
  1. Eng, L.F. et al. (2000) Neurochem. Res. 25, 1439.
  2. Herrmann H and Aebi, U. (1998) Curr. Opin. Struct. Biol. 8, 177.
  3. Yang, Z. et al. (2015) Trends Neurosci. 38, 364.
  4. Prust, M. et al. (2011) Neurology 77, 1287.
  5. Kamphuis, W. et al. (2014) Neurobiol. Aging 35, 492.
Scientific background

Glial fibrillary acidic protein (GFAP) is a key intermediate filament (IF) III protein responsible for maintaining the mechanical strength of glia cells by supporting their cytoskeleton structure. GFAP is expressed in astrocytes in the CNS, non-myelinating Schwann cells in the PNS, and enteric glial cells1.

GFAP has a structural organization that is typical to class III IF proteins: it has a head, rod, and tail domains. The N-terminal head domain is important for filament formation and the C-terminal domain is important for oligomerization2.

GFAP is encoded by a single gene mapped to human chromosome 17q21. To date, 10 isoforms/splice variants have been identified. GFAP is tightly regulated: both at mRNA transcription level and by phosphorylation and other post-translational modifications. A number of growth factors such as CNTFFGF and TGF-β can induce GFAP gene transcription activation leading to increased GFAP protein levels3.

Single nucleotide polymorphism (SNP) in GFAP results in the formation of Rosenthal fibers that cause Alexander Disease, hence, GFAP is a potential drug target for the treatment of this disease. A number of GFAP mutations were found in the coding and in the promoter regions of Alexander disease patients4.

GFAP gene activation and protein induction appear to play a critical role in astroglia cell activation (astrogliosis) following CNS injuries and neurodegeneration. GFAP protein and its breakdown products are rapidly released into biofluids following traumatic brain and spinal cord injuries and stroke, making them strong candidate biomarkers for such neurological disorders5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 22/08/2021

Guinea Pig Anti-GFAP Antibody (#AGP-307) is a highly specific antibody directed against an epitope of the rat Glial fibrillary acidic protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GFAP from rat and mouse samples. The antibody will not recognize GFAP from human samples.

The antigen used to immunize guinea pigs is the same as Anti-GFAP Antibody (#AFP-001) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.

For research purposes only, not for human use
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